Peer-Reviewed Journal Details
Mandatory Fields
Goradia, D; Cooney, J; Hodnett, BK; Magner, E
2006
August
Biotechnology Progress
Characteristics of a mesoporous silicate immobilized trypsin bioreactor in organic media
Published
()
Optional Fields
CATALYZED PEPTIDE-SYNTHESIS ENZYMATIC CATALYSIS COSOLVENT MIXTURES NONAQUEOUS MEDIA SOLVENTS ENZYMES WATER STABILITY OXIDATION PROTEASES
22
4
1125
1131
Mesoporous silicates ( MPS) materials are attractive materials for immobilizing proteins/enzymes because of their well ordered structures, large surface areas (up to 1000 m(2) g(-1)), narrow pore size distributions, large pore diameters, and pore volumes. MPS with average pore diameters ranging from 28 to 300 angstrom were prepared using cationic and nonionic surfactants. The influence of water content, pH, storage, and thermal treatment on the activity of trypsin immobilized onto MPS was investigated. In a range of solvents, the amidolytic activity of immobilized trypsin was higher than that of the lyophilized preparation. Significant increases in k(cat)/K-M occurred in propanol, ethanol, methanol, and formamide of 90, 62, 45 and 26, respectively. The observed increases were primarily a result of substantial increases in k(cat).
8756-7938
10.1021/bp050334y
Grant Details